African Journal of Paediatric Surgery

ORIGINAL ARTICLE
Year
: 2022  |  Volume : 19  |  Issue : 2  |  Page : 78--82

Paediatric osteoarticular infections caused by staphylococcus aureus producing panton–valentine leucocidin in morocco: Risk factors and clinical features


Kaoutar Moutaouakkil1, Hicham Abdellaoui2, Btissam Arhoune1, Karima Atarraf2, Samira El Fakir3, Ghita Yahyaoui4, Mustapha Mahmoud4, Moulay Abderrahmane Afifi2, Bouchra Oumokhtar1 
1 Microbiology and Molecular Biology Laboratory, Faculty of Medicine and Pharmacy, Sidi Mohammed Ben Abdellah University, Fez, Morocco
2 Pediatric Orthopedic Traumatology Department, Hassan II University Teaching Hospital, Faculty of Medicine and Pharmacy, Sidi Mohammed Ben Abdellah University, Fez, Morocco
3 Laboratory of Epidemiology, Clinical Research and Community Health- Faculty of Medicine and Pharmacy, Sidi Mohammed Ben Abdellah University, Fez, Morocco
4 Central Laboratory of Medical Analysis, Hassan II University Teaching Hospital, Faculty of Medicine and Pharmacy, Sidi Mohammed Ben Abdellah University, Fez, Morocco

Correspondence Address:
Prof. Bouchra Oumokhtar
Microbiology and Molecular Biology Laboratory, Faculty of Medicine and Pharmacy, Sidi Mohammed Ben Abdellah University, Fez
Morocco

Objective: We aimed to estimate the prevalence of Staphylococcus aureus producing Panton–Valentine leucocidin (PVL) isolated from children diagnosed with osteoarticular infections (OAIs), and to examine risk factors and clinical features. Methods: This prospective study was conducted from January 2017 to December 2018. All hospitalised children diagnosed with S. aureus OAI are included. Blood cultures, articular fluids, synovial tissues and/or bone fragments were collected for bacteriological culture. Antimicrobial susceptibility tests were determined by disk diffusion method. Genes encoding methicillin resistance (mecA) and PVL virulence factors (luk-S-PV and luk-F-PV) were detected by multiplex polymerase chain reaction. The demographic, clinical, laboratory, radiographic and clinical features were reviewed prospectively from medical records. Results: A total of 37 children with S. aureus OAIs were included, 46% of them have PVL-positive infection and 70.6% were male. The mean age was 8.12 years (±4.57), and almost were from rural settings (76.5%). Children with Staphylococcus aureus producing Panton–Valentine leucocidin (SA-PVL) were significantly associated with type of infection (P = 0.005), location of infection (P = 0.037) and abnormal X-ray (P = 0.029). All strains SA-PVL+ are sensitive to methicillin, but one strain SA-PVL negative was methicillin-resistant S. aureus, confirmed by gene mecA positive. Conclusion: The prevalence of S. aureus infections producing PVL toxin was high in OAIs amongst Moroccan children, mainly due to methicillin-susceptible S. aureus. Type and location of infections and abnormal X-ray were significantly associated with SA-PVL. Routine diagnostic testing of PVL-SA, continuous epidemiological surveillance and multidisciplinary management of OAI is essential to prevent serious complications.


How to cite this article:
Moutaouakkil K, Abdellaoui H, Arhoune B, Atarraf K, El Fakir S, Yahyaoui G, Mahmoud M, Afifi MA, Oumokhtar B. Paediatric osteoarticular infections caused by staphylococcus aureus producing panton–valentine leucocidin in morocco: Risk factors and clinical features.Afr J Paediatr Surg 2022;19:78-82


How to cite this URL:
Moutaouakkil K, Abdellaoui H, Arhoune B, Atarraf K, El Fakir S, Yahyaoui G, Mahmoud M, Afifi MA, Oumokhtar B. Paediatric osteoarticular infections caused by staphylococcus aureus producing panton–valentine leucocidin in morocco: Risk factors and clinical features. Afr J Paediatr Surg [serial online] 2022 [cited 2022 Dec 1 ];19:78-82
Available from: https://www.afrjpaedsurg.org/article.asp?issn=0189-6725;year=2022;volume=19;issue=2;spage=78;epage=82;aulast=Moutaouakkil;type=0